Part:BBa_M50089:Design

GadB

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 652
  Illegal EcoRI site found at 1090
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 652
  Illegal EcoRI site found at 1090
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 652
  Illegal EcoRI site found at 1090
  Illegal BamHI site found at 567
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 652
  Illegal EcoRI site found at 1090
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 652
  Illegal EcoRI site found at 1090
  Illegal NgoMIV site found at 1218
  Illegal AgeI site found at 480
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The sequence was optimized for E. coli K-12 using CodonOpt.

Source

The gene is sourced from E. coli. The GadB gene sequence was obtained from UniProt (Accession Number: P69910).

References

http://www.uniprot.org/uniprot/P69910

https://biocyc.org/gene?orgid=ECOLI&id=EG11490

Harden, M. M., He, A., Creamer, K., Clark, M. W., Hamdallah, I., Martinez II, K. A.,Slonczewski, J. L. (2015). Acid-adapted strains of escherichia coli K-12 obtained by experimental evolution. Applied and Environmental Microbiology, 81(6), 1932-1941. doi:10.1128/AEM.03494-14

Krulwich, T. A., Sachs, G., & Padan, E. (2011). Molecular aspects of bacterial pH sensing and homeostasis Nature Reviews: Microbiology, 9, 330-343. doi:10.1038/nrmicro2549

Ma, Z., Gong, S., Richard, H., Tucker, D. L., Conway, T., & Foster, J. W. (2003). GadE (YhiE) activates glutamate decarboxylase-dependent acid resistance in escherichia coli K-12. Molecular Microbiology, 49(5), 1309-1320. doi:10.1046/j.1365-2958.2003.03633.

H. Richard, & J.W., F. (2004). Escherichia coli glutamate- and arginine-dependent acid resistance systems increase internal pH and reverse transmembrane potential. Journal of Bacteriology, 186(18), 6032-6041. doi:10.1128/JB.186.18.6032-6041.2004